Inhibitory effect of clove methanolic extract and eugenol on dendritic cell functions

Ching-Hsiung Lin; Sheng Hao Lin; Chi-Chen Lin; Yi-Chen Liu; Chao-Jung Chen; Ching-Liang Chu; Hui-Chi Huang; Ming-Kuem Lin

Journal of Functional Foods (Dec 2016)

Inhibitory effect of clove methanolic extract and eugenol on dendritic cell functions

Ching-Hsiung Lin,
Sheng Hao Lin,
Chi-Chen Lin,
Yi-Chen Liu,
Chao-Jung Chen,
Ching-Liang Chu,
Hui-Chi Huang,
Ming-Kuem Lin

Affiliations

Ching-Hsiung Lin: Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, 135 Nanxiao St., Changhua City, Changhua County 500, Taiwan
Sheng Hao Lin: Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, 135 Nanxiao St., Changhua City, Changhua County 500, Taiwan; Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, 145 Xingda Rd., South Dist., Taichung 402, Taiwan
Chi-Chen Lin: Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, 135 Nanxiao St., Changhua City, Changhua County 500, Taiwan; Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, 145 Xingda Rd., South Dist., Taichung 402, Taiwan; Department of Medical Research and Education, Taichung Veterans General Hospital, 1650 Taiwan Boulevard Sect. 4, Taichung 40705, Taiwan
Yi-Chen Liu: Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, 145 Xingda Rd., South Dist., Taichung 402, Taiwan; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Biopharmaceutical and Food Sciences, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan
Chao-Jung Chen: Graduate Institute of Integrated Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan; Department of Medical Research, China Medical University Hospital, 91 Hsueh-Shih Road, Taichung 40402, Taiwan
Ching-Liang Chu: Graduate Institute of Immunology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd, Taipei 100, Taiwan
Hui-Chi Huang: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Biopharmaceutical and Food Sciences, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan
Ming-Kuem Lin: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Biopharmaceutical and Food Sciences, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan; Corresponding author. Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan. Fax: +886 4 04 22070439.

Journal volume & issue: Vol. 27
pp. 439 – 447

Abstract

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Caryophyllata Flos or clove is widely used as a condiment and in Chinese medicine. It reportedly helps relieve asthma and allergies; however, the underlying mechanisms remain unclear. In particular, the mechanism affecting dendritic cells (DCs), which play a critical role in the immune response, remains unknown. In this study, we examine the effects of Caryophyllata Flos methanolic extract (CFME) and its major compound, eugenol, on DC functions. Our results showed that CFME and eugenol significantly inhibited DC activation and maturation. The IC50s of CFME and eugenol were approximately 25 µg/mL and 50 µM, respectively. CFME and eugenol halted T-cell proliferation. Contact hypersensitivity responses were inhibited in mice cosensitized with either CFME or eugenol. We demonstrated for the first time that clove and its major active ingredient, eugenol, exhibited a significant immunosuppressive effect on DC functions, revealing that clove is a functional food that can ameliorate chronic inflammation and autoimmunity.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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