Pretreatment and treatment with fructo-oligosaccharides attenuate intestinal mucositis induced by 5-FU in mice

Abstract

Mucositis is a side effect observed in patients undergoing chemotherapy. Besides, this treatment also leads to the imbalance of the intestinal microbiota. This study evaluated the effects of pre and treatment with fructo-oligosaccharides (FOS) in intestinal mucositis induced by 5-fluorouracil (5-FU). Balb/c mice were divided into four groups: CTL (without mucositis + saline), MUC (mucositis + saline), PT (mucositis + 6% FOS before disease induction) and T (mucositis + 6% FOS after disease induction). Mucositis was induced with an intraperitoneal injection of 300 mg/kg 5-FU. After 72 h intestinal permeability (IP), inflammatory infiltrate, oxidative stress, histological analysis, and short-chain fatty acids (SCFA) production were evaluated. The MUC group showed lost weight, intense inflammatory infiltrate, increased oxidative stress and IP (P < 0.05). FOS supplementation attenuated all these parameters (P < 0.05). Only pretreatment was able to maintain the acetate and butyrate production. These results showed that FOS supplementation presented protective effects on intestinal barrier function.

Keywords

• Mucositis
• 5-fluorouracil
• Fructo-oligosaccharides
• Intestinal permeability