PHENOLIC COMPOUNDS COMPOSITION OF LEONURUS QUINQUELOBATUS GILIB. HERB FROM DIFFERENT REGIONS OF WESTERN SIBERIA

Abstract

The main phenol compounds composition (flavonoids, phenolcarboxylic acids and its derivatives) and their ratio in Leonurus quinquelobatus Gilib. herb from Kemerovo region, regions with less developed chemical and coalmining industries (Novosibirsk and Omsk regions) and environmentally friendly areas (Altai Republic) were studied in 2011 for developing the influence of anthropogenic pollution on the quality of raw motherwort. The primary purification method and analysis by HPLC-MS of the L. quinquelobatus herb alcoholic extracts (70 %) was developed. Passed through a patron filled with phase reversed sorbent (Diapak C16), methanol furnished the best extract of flavonoids and hydroxycinnamic acids. An eluent was used for separation: 2 % formylic acid-methanol in the stepwise elution mode (isocratic and gradient modes combination). The 9 main phenolic compounds were found in the samples, 5 of them are flavonoids and their derivates (chlorogenic acid, ether coffeic and malic acid, tetrosopentozide chlorogenic acid, tetrosodipentozide chlorogenic acid, rutin, hexosokumaroilluteolin, quinquelozide isomers, methyl apigenin ether). A compound composition of the plants from different regions of Western Siberia was not different. The high content of rutin and chlorogenic acids in L. quinquelobatus medications did not demonstrate the high quality of the analysed raw material because the amount of these compounds can increase if the plants are damaged by diseases and blasts. Comparison of the quality of the L. quinquelobatus samples should beter be carried out by apigenin derivatives (including quinquelozide).

Keywords

• leonurus quinquelobatus gilib.
• motherwort
• hpls-ms
• flavonoids
• phenolcarboxylic acids
• phenolic compounds