In Situ Generation of Substrate via Bi-Potential Screen-Printed Electrode for Determination of Antioxidant Using Electrochemiluminescence

Abstract

Excess generation of reactive oxygen species (ROS) could trigger a number of human diseases. We focused that antioxidants in beverages suppress the effect of ROS. Electrochemiluminescence (ECL) was determined by newly designed bi-potential screen printed electrodes and electron multiplying charge coupled device (EM-CCD) camera. Luminol based ECL was quenched due to the consumption of ROS by the antioxidants. Rapid and sensitive detection of antioxidants such as ascorbic acid and chlorogenic acid were demonstrated in beverage samples.

Keywords

• electrochemiluminescence
• antioxidant
• screen-printed-electrode
• food analysis